Description of the "Temperature Scan with Changer for Decay Measurement" script
Short explanation of operation:
The script increases the temperature of the sample holder
in steps, and measures a decay histogram for each temperature.
A delay can be introduced after reaching the set temperature of the
sample holder to ensure optimal temperature equilibrium also in the cuvette.
A simple intensity optimizer ensures that the given pile up ratio
is not violated.
The sample changer is used to measure an instrument response function for
each temperature.
Hardware prerequisites:
FluoTime300 with
- Sample Changer Turret 400 (4 position cuvette holder with temperature control)
- Detection polarizer
- DETECTOR_1 (used for measurement)
Note: It is possible to use use DETECTOR_2 for the measurement.
Replace all occurrences of DETECTOR_1 in the script by DETECTOR_2
Software prerequisites:
EasyTau Version > 1.1
Prior running the script
Please adapt the script to your needs
Please set manually:
Emission monochromator exit port to deliver light to DETECTOR_1
- Emission monochromator slit width / bandwidth to define the detection bandpass
- Electrical switches (if exist) to deliver electrical signal from DETECTOR_1 to TCSPC electronics
- Laser settings wavelength, repetition frequency, intensity
- TCSPC electronics for measurement
- TCSPC electronics bin width
Values to adjust in the script:
- Sample: Sample name for workspace
- Solvent: Solvent name for workspace
- SamplePosition: Position of sample in the sample changer
- SamplePosition: Detection wavelength given in nanometer
- ScatterPosition: Position of scatter solution in the sample changer
- IRFDetectionWL: Laser IRF wavelength given in nanometer
- StartTemp: Start of temperature ramp in °C
- StopTemp: End of temperature ramp in °C
- TempStep: Step size for temperature ramp in °C
- EqDelay: Delay in seconds to wait for temp. equilibrium in the sample